"Yes, rats can indeed ""laugh,"" although it is not audible to humans. Research has shown that when rats, especially juvenile ones, are tickled or engage in rough-and-tumble play, they emit ultrasonic vocalizations (USVs) at around 50 kHz, which is well above the threshold of human hearing (typically up to 20–20.5 kHz). Studies conducted by Jaak Panksepp and colleagues found that when rats are tickled on their belly or nape, they not only emit these high-frequency chirps but also display playful